| 000 | 03438nam a22005535i 4500 | ||
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| 001 | 978-981-10-4753-4 | ||
| 003 | DE-He213 | ||
| 005 | 20220801213615.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 170720s2018 si | s |||| 0|eng d | ||
| 020 |
_a9789811047534 _9978-981-10-4753-4 |
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| 024 | 7 |
_a10.1007/978-981-10-4753-4 _2doi |
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| 050 | 4 | _aR856-857 | |
| 072 | 7 |
_aMQW _2bicssc |
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| 072 | 7 |
_aTEC059000 _2bisacsh |
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| 072 | 7 |
_aMQW _2thema |
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| 082 | 0 | 4 |
_a610.28 _223 |
| 100 | 1 |
_aZhuang, Bin. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _933162 |
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| 245 | 1 | 0 |
_aDevelopment of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis _h[electronic resource] / _cby Bin Zhuang. |
| 250 | _a1st ed. 2018. | ||
| 264 | 1 |
_aSingapore : _bSpringer Nature Singapore : _bImprint: Springer, _c2018. |
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| 300 |
_aXXIII, 114 p. 58 illus., 44 illus. in color. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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_acomputer _bc _2rdamedia |
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_aonline resource _bcr _2rdacarrier |
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_atext file _bPDF _2rda |
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_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5061 |
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| 505 | 0 | _aIntroduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work. | |
| 520 | _aThis thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice. | ||
| 650 | 0 |
_aBiomedical engineering. _93292 |
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| 650 | 0 |
_aBiotechnology. _97533 |
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| 650 | 0 |
_aBiomaterials. _916365 |
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| 650 | 0 |
_aNucleic acids. _933163 |
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| 650 | 1 | 4 |
_aBiomedical Engineering and Bioengineering. _931842 |
| 650 | 2 | 4 |
_aChemical Bioengineering. _933164 |
| 650 | 2 | 4 |
_aNucleic Acid. _933165 |
| 710 | 2 |
_aSpringerLink (Online service) _933166 |
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| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9789811047527 |
| 776 | 0 | 8 |
_iPrinted edition: _z9789811047541 |
| 776 | 0 | 8 |
_iPrinted edition: _z9789811352201 |
| 830 | 0 |
_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5061 _933167 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-981-10-4753-4 |
| 912 | _aZDB-2-ENG | ||
| 912 | _aZDB-2-SXE | ||
| 942 | _cEBK | ||
| 999 |
_c75384 _d75384 |
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