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001			9781351132954
003			FlBoTFG
005			20220711212856.0
006			m o d
007			cr
008			181122s2018 fluab ob 001 0 eng d
020			_a9781351132954 _q(e-book : PDF)
035			_a(OCoLC)1022757089
040			_aFlBoTFG _cFlBoTFG _erda
041	1		_aeng
050		4	_aQK570.2
072		7	_aMED _x009000 _2bisacsh
072		7	_aTEC _x010000 _2bisacsh
072		7	_aSCI _x039000 _2bisacsh
072		7	_aTCB _2bicscc
082	0	4	_aSCEC5010 _223
245	0	0	_aProtocols for Macroalgae Research / _cedited by Bénédicte Charrier, Thomas Wichard and C R K Reddy.
250			_aFirst edition.
264		1	_aBoca Raton, FL : _bCRC Press, _c2018.
300			_a1 online resource (518 pages) : _b121 illustrations, text file, PDF
336			_atext _2rdacontent
337			_acomputer _2rdamedia
338			_aonline resource _2rdacarrier
504			_aIncludes bibliographical references and index.
505	0	0	_tCONTENTS -- _tSection I: Cultivating and preserving seaweeds -- _tChapter 1 Seaweed in high-energy environments: Protocol -- _tto move Saccharina cultivation offshore----Bela H. Buck and Britta Grote -- _tChapter 2 Cultivation protocol for Saccharina latissima -- _tSilje Forbord, Kristine Braaten Steinhovden, -- _tKaia Kjlbo Rd, Aleksander Hand, and Jorunn Skjermo -- _tChapter 3 Derivation of clonal stock cultures and hybridization -- _tof kelps: A tool for strain preservation and breeding -- _tprograms----Inka Bartsch -- _tChapter 4 Cryopreservation of macroalgae----John G. Day -- _tChapter 5 Unraveling seaweeds bacteriomes: From field site -- _tto computer screen----Tnia Aires, Gerard Muyzer, Ester A. Serro, and -- _tAschwin H. Engelen -- _tChapter 6 Heavy metal ecotoxicity on the early life history -- _tstages of macroalgae----Pablo P. Leal and Michael Y. Roleda -- _tChapter 7 A simple protocol for a rapid and consistent -- _tproduction of a large number of viable protoplasts -- _tfrom the Ulvophycean species----Vishal Gupta and C.R.K. Reddy -- _tChapter 8 Purification of sporulation and swarming inhibitors -- _tfrom Ulva: Application in algal life-cycle controlling----Ralf W. Kessler, Taghreed Alsufyani, and Thomas Wichard -- _tChapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)----Gianmaria Califano and Thomas Wichard -- _tSection II: Chemical composition -- _tChapter 10 Biochar production from seaweeds----Loretto Contreras-Porcia, Matas Araya, -- _tElizabeth Garrido-Ramrez, Cristian Bulboa, -- _tJean Pierre Remonsellez, Javier Zapata, Camila Espinoza, -- _tand Jorge Rivas -- _tChapter 11 Identification and quantification of laminarins -- _tin brown algae----Angelika Graiff, Wolfgang Ruth, and Ulf Karsten -- _tChapter 12 Determination of carbohydrate composition -- _tof macroalgae----Wouter J.J. Huijgen, E.M. Cobussen-Pool, B.F. van Egmond, -- _tand J.W. van Hal -- _tChapter 13 Quantification of proteins in seaweeds----Carl Safi, Jelle van Leeuwen, Yvette Telleman, -- _tNicole Engelen-Smit, Lambertus van den Broek, -- _tand Paulien Harmsen -- _tChapter 14 Comprehensive phytohormone quantification -- _tin the red alga Pyropia yezoensis by liquid -- _tchromatographymass spectrometry----Takakazu Matsuura, Izumi C. Mori, Yoko Ikeda, -- _tTakashi Hirayama, and Koji Mikami -- _tChapter 15 Total phenolic content and antioxidant capacity -- _tanalysis of seaweed biomass----Xiaoru Hou, Randi Neerup, and Anne-Belinda Bjerre -- _tChapter 16 Extraction of phycocyanin and phycoerythrin pigments----Stewart William Beattie, Michle Moranais, Paul Dlris, -- _tJol Fleurence, and Justine Dumay -- _tChapter 17 Quantification and localization of reactive oxygen -- _tspecies in marine macrophytes----Manoj Kumar, Loretto Contreras-Porcia, Nirali M. Kumar, -- _tand Peter J. Ralph -- _tChapter 18 Metabolomics of intra- and extracellular metabolites -- _tfrom micro- and macroalgae using GCMS -- _tand LCMS----Constanze Kuhlisch, Gianmaria Califano, Thomas Wichard, -- _tand Georg Pohnert -- _tChapter 19 Preparative extraction of exometabolites -- _tfrom seaweed surfaces----Florian Weinberger -- _tChapter 20 Disruption-free solid-phase extraction of surface -- _tmetabolites from macroalgae----Emilio Cirri and Georg Pohnert -- _tSection III: Cellular and molecular characterization -- _tChapter 21 The immunodetection and in situ imaging of -- _tcell-wall polysaccharides in brown algae----Amandine Simon, Delphine Duffieux, Ccile Herv, -- _tSophie Le Panse, Paul Knox, and Thomas Torode -- _tChapter 22 Atomic force microscopy based analysis of cell-wall -- _telasticity in macroalgae----Thomas Torode, Marina Linardic, J. Louis Kaplan, -- _tand Siobhan A. Braybrook -- _tChapter 23 Dynamic and microscale mapping of cell growth: -- _tCase of Ectocarpus filament cells----Herv Rabill, Bernard Billoud, Elodie Rolland, -- _tand Bndicte Charrier -- _tChapter 24 Actin fluorescent staining in the filamentous brown -- _talga Ectocarpus siliculosus -- _tHerv Rabill, Maria Koutalianou, Bndicte Charrier, -- _tand Christos Katsaros -- _tChapter 25 Cryofixation of brown algae for transmission -- _telectron microscopy----Chikako Nagasato, Christos Katsaros, and Taizo Motomura -- _tChapter 26 Probing the subcellular topography of -- _tseaweeds: Transmission electron microscopy, -- _timmunocytochemistry, and correlative light -- _tmicroscopy----Sandra C. Raimundo and David S. Domozych -- _tChapter 27 Coralline algae preparation for scanning electron -- _tmicroscopy and optical microscopy----S. Kaleb, G. Alongi, and A. Falace -- _tChapter 28 Extraction of high quality RNA from brown algae -- _tfor transcriptomic analysis----Sandra Heinrich -- _tChapter 29 Induction of sexual reproduction in Spirogyra -- _tcultures for laser capture microdissection of gametes -- _tand zygotes----Denis Saint-Marcoux and Jane A. Langdale -- _tChapter 30 Cloning and expression strategies for the -- _tpostgenomic analysis of brown algae----Agns Groisillier -- _tChapter 31 Polyethylene glycol-mediated transformation in -- _tthe green macroalga Ulva mutabilis (Chlorophyta): -- _tA forward genetics approach----Jens Boesger, Michiel Kwantes, and Thomas Wichard -- _tIndex.
520	3		_aProtocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids. Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing　fundamental　research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures, which include helpful notes offering care and cautions.　This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.
530			_aAlso available in print format.
650		7	_aTECHNOLOGY & ENGINEERING / Environmental / General. _2bisacsh _95118
650		7	_aSCIENCE / Life Sciences / Biology / Marine Biology. _2bisacsh _920392
650		0	_aMarine algae _xResearch _xPopular works. _920393
650		0	_aSCIENCE _xLife Sciences _xBiology. _920394
650		0	_aSCIENCE _xLife Sciences _xMicrobiology. _920395
650		0	_aMarine algae _xResearch. _920396
655		7	_aElectronic books. _93294
700	1		_aCharrier, Bénédicte, _eeditor. _920397
700	1		_aWichard, Thomas, _eeditor. _920398
700	1		_aReddy, C R K, _eeditor. _920399
710	2		_aTaylor and Francis. _910719
776	0	8	_iPrint version: _z9781498796422
856	4	0	_uhttps://www.taylorfrancis.com/books/9781351132954 _zClick here to view.
942			_cEBK
999			_c72370 _d72370