Protocols for Macroalgae Research /
edited by Bénédicte Charrier, Thomas Wichard and C R K Reddy.
- First edition.
- 1 online resource (518 pages) : 121 illustrations, text file, PDF
Includes bibliographical references and index.
CONTENTS -- Section I: Cultivating and preserving seaweeds -- Chapter 1 Seaweed in high-energy environments: Protocol -- to move Saccharina cultivation offshore----Bela H. Buck and Britta Grote -- Chapter 2 Cultivation protocol for Saccharina latissima -- Silje Forbord, Kristine Braaten Steinhovden, -- Kaia Kjlbo Rd, Aleksander Hand, and Jorunn Skjermo -- Chapter 3 Derivation of clonal stock cultures and hybridization -- of kelps: A tool for strain preservation and breeding -- programs----Inka Bartsch -- Chapter 4 Cryopreservation of macroalgae----John G. Day -- Chapter 5 Unraveling seaweeds bacteriomes: From field site -- to computer screen----Tnia Aires, Gerard Muyzer, Ester A. Serro, and -- Aschwin H. Engelen -- Chapter 6 Heavy metal ecotoxicity on the early life history -- stages of macroalgae----Pablo P. Leal and Michael Y. Roleda -- Chapter 7 A simple protocol for a rapid and consistent -- production of a large number of viable protoplasts -- from the Ulvophycean species----Vishal Gupta and C.R.K. Reddy -- Chapter 8 Purification of sporulation and swarming inhibitors -- from Ulva: Application in algal life-cycle controlling----Ralf W. Kessler, Taghreed Alsufyani, and Thomas Wichard -- Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)----Gianmaria Califano and Thomas Wichard -- Section II: Chemical composition -- Chapter 10 Biochar production from seaweeds----Loretto Contreras-Porcia, Matas Araya, -- Elizabeth Garrido-Ramrez, Cristian Bulboa, -- Jean Pierre Remonsellez, Javier Zapata, Camila Espinoza, -- and Jorge Rivas -- Chapter 11 Identification and quantification of laminarins -- in brown algae----Angelika Graiff, Wolfgang Ruth, and Ulf Karsten -- Chapter 12 Determination of carbohydrate composition -- of macroalgae----Wouter J.J. Huijgen, E.M. Cobussen-Pool, B.F. van Egmond, -- and J.W. van Hal -- Chapter 13 Quantification of proteins in seaweeds----Carl Safi, Jelle van Leeuwen, Yvette Telleman, -- Nicole Engelen-Smit, Lambertus van den Broek, -- and Paulien Harmsen -- Chapter 14 Comprehensive phytohormone quantification -- in the red alga Pyropia yezoensis by liquid -- chromatographymass spectrometry----Takakazu Matsuura, Izumi C. Mori, Yoko Ikeda, -- Takashi Hirayama, and Koji Mikami -- Chapter 15 Total phenolic content and antioxidant capacity -- analysis of seaweed biomass----Xiaoru Hou, Randi Neerup, and Anne-Belinda Bjerre -- Chapter 16 Extraction of phycocyanin and phycoerythrin pigments----Stewart William Beattie, Michle Moranais, Paul Dlris, -- Jol Fleurence, and Justine Dumay -- Chapter 17 Quantification and localization of reactive oxygen -- species in marine macrophytes----Manoj Kumar, Loretto Contreras-Porcia, Nirali M. Kumar, -- and Peter J. Ralph -- Chapter 18 Metabolomics of intra- and extracellular metabolites -- from micro- and macroalgae using GCMS -- and LCMS----Constanze Kuhlisch, Gianmaria Califano, Thomas Wichard, -- and Georg Pohnert -- Chapter 19 Preparative extraction of exometabolites -- from seaweed surfaces----Florian Weinberger -- Chapter 20 Disruption-free solid-phase extraction of surface -- metabolites from macroalgae----Emilio Cirri and Georg Pohnert -- Section III: Cellular and molecular characterization -- Chapter 21 The immunodetection and in situ imaging of -- cell-wall polysaccharides in brown algae----Amandine Simon, Delphine Duffieux, Ccile Herv, -- Sophie Le Panse, Paul Knox, and Thomas Torode -- Chapter 22 Atomic force microscopy based analysis of cell-wall -- elasticity in macroalgae----Thomas Torode, Marina Linardic, J. Louis Kaplan, -- and Siobhan A. Braybrook -- Chapter 23 Dynamic and microscale mapping of cell growth: -- Case of Ectocarpus filament cells----Herv Rabill, Bernard Billoud, Elodie Rolland, -- and Bndicte Charrier -- Chapter 24 Actin fluorescent staining in the filamentous brown -- alga Ectocarpus siliculosus -- Herv Rabill, Maria Koutalianou, Bndicte Charrier, -- and Christos Katsaros -- Chapter 25 Cryofixation of brown algae for transmission -- electron microscopy----Chikako Nagasato, Christos Katsaros, and Taizo Motomura -- Chapter 26 Probing the subcellular topography of -- seaweeds: Transmission electron microscopy, -- immunocytochemistry, and correlative light -- microscopy----Sandra C. Raimundo and David S. Domozych -- Chapter 27 Coralline algae preparation for scanning electron -- microscopy and optical microscopy----S. Kaleb, G. Alongi, and A. Falace -- Chapter 28 Extraction of high quality RNA from brown algae -- for transcriptomic analysis----Sandra Heinrich -- Chapter 29 Induction of sexual reproduction in Spirogyra -- cultures for laser capture microdissection of gametes -- and zygotes----Denis Saint-Marcoux and Jane A. Langdale -- Chapter 30 Cloning and expression strategies for the -- postgenomic analysis of brown algae----Agns Groisillier -- Chapter 31 Polyethylene glycol-mediated transformation in -- the green macroalga Ulva mutabilis (Chlorophyta): -- A forward genetics approach----Jens Boesger, Michiel Kwantes, and Thomas Wichard -- Index.
Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids. Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures, which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.